Chibane Mohamed, Bouchra Dahmoune, Amina Dahmoune and Merzouk Mamou
The Management Laboratory and Development of Natural Resources and Quality Assurance, AMO University Bouira, Algeria
Our work focuses on the evaluation of the hemolytic activity of saponin (steroidal glycoside) isolated from the starfish Echinaster sepositus of the Algerian coast, and the determination of their heterogeneity inter-body distribution. The bodywall (aboral face and oral face), gonads, stomach and pyloric caeca are the five organs selected for analysis. From the in vitro hemolytic tests, we have noted that the hemolytic performance of the five extracts is different. The gonads′ and bodywall saponins are the most active in blood red cells. With law hemolytic rate of about 99,989% and 99.86% respectively.
This difference in the toxic effect of different saponins of Echinaster sepositus can be attributed to their chemical structure which reflects the existence of large diversity of chemical molecules of saponins and an important heterogeneity inter-body. The analysis of different extracts by high performance liquid chromatography (CLHP) shows that the five extracts have three majority and similar peaks located between 3.3 min and 7 .8 min and a minor peak at 5 min. Which indicate the presence of three major types of isomers which have a very high qualitative and quantitative similarity in the five saponins extracts. The gonads′s and bodywall chromatograms show a strong similarity with a fourth important peak at 16 min. Which may indicate the presence of particular isomer saponins, this isomer is specific to the gonads and bodywall. For the bodywall (aboral face) is observed two peaks located at 10 min and 11 min respectively. Which indicate the presence of two other isomers specific to the bodywall (aboral face). The specific isomers located at 16 min, 11 min, 10 min are responsible for the high toxicity of the gonads′ and bodywall saponins.
Keywords: Steroidal glycoside, saponin, Starfish, Echinaster sepositus, hemolytic activity, Algerian Coast, CLHP analysis.