Jasem Saki, Shahram Khademvatan, Nader Pazyar, Alborz Eskandari, Ali Tamoradi, Pedram
Department of Parasitology, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
Background: Conventional treatments against leishmaniasis have several side effects and disadvantages such as drug resistance. There is necessary demand to achieve treatments that are more effective.
Objective: The aim of this investigation was to determine anti-leishmanial effects of Cordia myxa mucilage extract on L. infantum, and L. major promastigotes under in vitro condition.
Methods: The serial concentrations of Cordia myxa mucilage extract were prepared with 0.61, 1.22, 2.44, 4.875, 9.75, 19.5, 39, 78 and 156 mg/ml w/v. The L. infantum and L. major promastigotes were treated with Cordia myxa mucilage extract for 72 h. Anti-leishmanial activity was assayed by light microscopic and (3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyl tetrazolium bromide) MTT methods.
Results: According to the study, Cordia myxa extract had a significant effect against L.major and L.infantum promastigotes dose dependently. Mucilage extract of Cordia myxa was active against promastigote form of L.major and L.infantum with the IC50 =26±2.2 mg/ml and 35±2.2 mg/ml respectively.
Conclusion: Cordia myxa extract might be a new anti-leishmanial agent; however, more studies are required to confirm it, especially in vivo condition.
Keywords: Cordia myxa, L.major, L.infantum, in vitro.