Mehdi Tavalla, Ebrahim Kazemi and Sharif Maraghi
Department of Parasitology, School of Medicine; Ahwaz Jundishapur University of Medical Sciences;
Microsporidiosis as opportunistic infections were frequently recognized in immunocompromised patients that cause Symptomatic or Asymptomatic infections, but can be severe and disseminated in immunosuppressed/immunocompromise (IS/IC) patients. The ribosomal RNAs complex with proteins to form two subunits, the large subunit (LSU) and small subunit (SSU). mRNA is sandwiched between the small and large subunits, and the ribosome catalyzes the formation of a peptide bond between the two amino acids that are contained in the rRNA. Fecal samples were collected from 186 patients, including 86 HIV+ and 100 cancer patients. In HIV+ patients, Microsporidia were detected in 27.9% (24/86) and 11.6% (10/86) and in cancer patients, 18% (18/100) and 7% (7/100) using PCR and Trichrome staining, respectively. Enterocytozoon bieneusi were found in 28 (66.7%) patients, higher than Encephalitozoon intestinalis, in 10 (23.8%) patients. Encephalitozoon cuniculi and Encephalitozoon hellem were detected in one and tree patients respectively. Microsporidia were significantly higher in HIV+ and cancer patients with diarrhea than those without diarrhea (P= 0.001). HIV+ individuals with Microsporidia, were significantly exhibit < 200 CD4 T cells/ μl blood (P= 0.001). Also there was significantly difference between contact with animals with positive results of HIV+ and cancer patients (p< 0.005). This study revealed that PCR technique was more sensitive than trichrome staining, also emphasize the importance of presence intestinal microsporidiosis as agent of diarrhea in HIV+ and cancer patients that can affect the therapy. Therefore early diagnosis of microsporidiosis would certainly be helpful in the management and implementation of treatment strategies in patients.
Keywords: Microsporidia, SSU-rRNA, PCR, HIV, Immunocompromise.