Kyung-Hyun Cho, Seong-Min Kim and Jeong-Ah Yoo
School of Biotechnology, Yeungnam University, Gyeongsan, Republic of Korea
Although breast milk is the best nutritional source for infant growth, however, there has been no information about the quality of breast milk from Trans fatty acid (TFA)-consumer. Compositional and functional analyses of breast milk in terms of lipid content and zebrafish embryo survivability. Control group showed 2.5- and 4.5-fold higher cholesterol content than TFA group and formula, respectively. The TFA group and formula showed 1.8- and 2.0-fold higher triglyceride (TG) than control group. TFA group and formula showed 1.4- and 4.8-fold higher glucose levels than that control. The TFA group also showed 25% lower protein content than control. Microinjection with breast milk from the TFA group showed significantly lower zebrafish embryo survivability compared to the control, whereas microinjection with formula showed the lowest survivability (39±5%) with the slowest developmental speed. Immunodetection revealed that breast milk from the TFA group showed smaller-sized apoA-I (25.5±0.6 kDa) than that from the control group (27.5±1.5 kDa), whereas formula did not contain apoA-I. Larger apoA-I size in breast milk was directly associated with higher embryo survivability. Breast milk from the TFA group showed increased TG and loss of cholesterol, lactalbumin (14 kDa), and apoA-I proteins, resulting in functional impairment of development and growth.